{"id":991085,"date":"2026-10-23T00:59:46","date_gmt":"2026-10-23T00:59:46","guid":{"rendered":"https:\/\/crei.vnua.edu.vn\/?p=991085"},"modified":"2026-10-23T00:59:46","modified_gmt":"2026-10-23T00:59:46","slug":"nghien-cuu-hoan-thien-quy-trinh-nhan-giong-in-vitro-cay-lan-kim-tuyen-tu-choi-nach","status":"publish","type":"post","link":"https:\/\/crei.vnua.edu.vn\/?p=991085","title":{"rendered":"Nghi\u00ean c\u1ee9u ho\u00e0n thi\u1ec7n quy tr\u00ecnh nh\u00e2n gi\u1ed1ng in vitro c\u00e2y lan kim tuy\u1ebfn t\u1eeb ch\u1ed3i n\u00e1ch"},"content":{"rendered":"<div class=\"vgblk-rw-wrapper limit-wrapper\">Lan kim tuy\u1ebfn (Anoectochilus lylei Rolfe ex Downie) l\u00e0 lo\u00e0i lan c\u00f3 gi\u00e1 tr\u1ecb, \u0111\u01b0\u1ee3c quan t\u00e2m trong nghi\u00ean c\u1ee9u v\u00e0 b\u1ea3o t\u1ed3n ngu\u1ed3n gen th\u1ef1c v\u1eadt. Vi\u1ec7c x\u00e2y d\u1ef1ng quy tr\u00ecnh nh\u00e2n gi\u1ed1ng hi\u1ec7u qu\u1ea3 c\u00f3 \u00fd ngh\u0129a quan tr\u1ecdng trong vi\u1ec7c ch\u1ee7 \u0111\u1ed9ng t\u1ea1o ngu\u1ed3n c\u00e2y gi\u1ed1ng, ph\u1ee5c v\u1ee5 c\u00f4ng t\u00e1c b\u1ea3o t\u1ed3n v\u00e0 ph\u00e1t tri\u1ec3n lo\u00e0i c\u00e2y n\u00e0y. Nghi\u00ean c\u1ee9u c\u1ee7a nh\u00f3m t\u00e1c gi\u1ea3 <em>V\u0169 Ng\u1ecdc Lan, Ph\u1ea1m Tu\u1ea5n Anh v\u00e0 Nguy\u1ec5n Th\u1ecb Ph\u01b0\u01a1ng Dung thu\u1ed9c<\/em> <em>Khoa N\u00f4ng h\u1ecdc, H\u1ecdc vi\u1ec7n N\u00f4ng nghi\u1ec7p Vi\u1ec7t Nam<\/em> \u0111\u00e3 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c \u0111i\u1ec1u ki\u1ec7n ph\u00f9 h\u1ee3p cho t\u1eebng giai \u0111o\u1ea1n nh\u00e2n gi\u1ed1ng <em>in vitro<\/em> c\u00e2y lan kim tuy\u1ebfn t\u1eeb ch\u1ed3i n\u00e1ch, g\u00f3p ph\u1ea7n ho\u00e0n thi\u1ec7n quy tr\u00ecnh s\u1ea3n xu\u1ea5t c\u00e2y gi\u1ed1ng b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y m\u00f4. X\u00e1c \u0111\u1ecbnh \u0111i\u1ec1u ki\u1ec7n kh\u1eed tr\u00f9ng m\u1eabu v\u00e0 t\u00e1i sinh ch\u1ed3i.<\/p>\n<table id=\"undefined\" class=\"imgEditor\" border=\"0\">\n<tbody>\n<tr>\n<td><img decoding=\"async\" class=\"imgtelerik\" src=\"https:\/\/file.vnua.edu.vn\/data\/0\/images\/2026\/10\/21\/host\/image001.png?w=680\" alt=\"\" \/><\/td>\n<\/tr>\n<tr class=\"alt_imgEditor\">\n<td>\u00a0<strong><em>C\u00e2y lan kim tuy\u1ebfn (S\u01b0u t\u1ea7m)<\/em><\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Nghi\u00ean c\u1ee9u \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n nh\u1eb1m ho\u00e0n thi\u1ec7n quy tr\u00ecnh nh\u00e2n gi\u1ed1ng <em>in vitro<\/em> c\u00e2y lan kim tuy\u1ebfn (Anoectochilus lylei Rolfe ex Downie). Th\u00ed nghi\u1ec7m \u0111\u01b0\u1ee3c b\u1ed1 tr\u00ed theo ki\u1ec3u ng\u1eabu nhi\u00ean ho\u00e0n to\u00e0n v\u1edbi 3 l\u1ea7n nh\u1eafc l\u1ea1i, qua \u0111\u00f3 \u0111\u00e1nh gi\u00e1 \u1ea3nh h\u01b0\u1edfng c\u1ee7a c\u00e1c \u0111i\u1ec1u ki\u1ec7n nu\u00f4i c\u1ea5y \u0111\u1ebfn kh\u1ea3 n\u0103ng t\u00e1i sinh, nh\u00e2n nhanh v\u00e0 ph\u00e1t tri\u1ec3n c\u1ee7a c\u00e2y.<\/p>\n<p>K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u x\u00e1c \u0111\u1ecbnh ch\u1ebf \u0111\u1ed9 kh\u1eed tr\u00f9ng k\u00e9p b\u1eb1ng dung d\u1ecbch Ca(OCl)\u2082 5% trong 10 ph\u00fat, k\u1ebft h\u1ee3p v\u1edbi H\u2082O\u2082 3% trong 5 ph\u00fat, l\u00e0 ph\u01b0\u01a1ng \u00e1n ph\u00f9 h\u1ee3p nh\u1ea5t trong c\u00e1c c\u00f4ng th\u1ee9c th\u00ed nghi\u1ec7m, cho t\u1ef7 l\u1ec7 m\u1eabu s\u1ed1ng \u0111\u1ea1t 98% sau 4 tu\u1ea7n nu\u00f4i c\u1ea5y.<\/p>\n<p>\u0110\u1ed1i v\u1edbi giai \u0111o\u1ea1n t\u1ea1o c\u1ee5m ch\u1ed3i, m\u00f4i tr\u01b0\u1eddng MS b\u1ed5 sung 20 g\/l sucrose, 6,5 g\/l agar, 30 ml\/l n\u01b0\u1edbc d\u1eeba, 2 mg\/l BAP v\u00e0 0,1 mg\/l IBA cho k\u1ebft qu\u1ea3 t\u1ed1t nh\u1ea5t. Sau 8 tu\u1ea7n nu\u00f4i c\u1ea5y, t\u1ef7 l\u1ec7 t\u1ea1o c\u1ee5m ch\u1ed3i \u0111\u1ea1t 81,33%, v\u1edbi trung b\u00ecnh 6,25 ch\u1ed3i\/c\u1ee5m. \u1ede giai \u0111o\u1ea1n nh\u00e2n nhanh, m\u00f4i tr\u01b0\u1eddng MS b\u1ed5 sung 20 g\/l sucrose, 6,5 g\/l agar, 30 ml\/l n\u01b0\u1edbc d\u1eeba, 1,0 mg\/l Kinetin v\u00e0 1,0 mg\/l IBA \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 ph\u00f9 h\u1ee3p nh\u1ea5t trong c\u00e1c c\u00f4ng th\u1ee9c kh\u1ea3o s\u00e1t. Sau 8 tu\u1ea7n nu\u00f4i c\u1ea5y, h\u1ec7 s\u1ed1 nh\u00e2n \u0111\u1ea1t 15,75 ch\u1ed3i\/m\u1eabu, chi\u1ec1u cao ch\u1ed3i trung b\u00ecnh \u0111\u1ea1t 3,35 cm.<\/p>\n<p>\u0110\u1ed1i v\u1edbi giai \u0111o\u1ea1n t\u1ea1o r\u1ec5, m\u00f4i tr\u01b0\u1eddng MS b\u1ed5 sung 6,5 g\/l agar, 20 g\/l sucrose v\u00e0 0,7 g\/l than ho\u1ea1t t\u00ednh cho t\u1ef7 l\u1ec7 t\u1ea1o r\u1ec5 \u0111\u1ea1t 100%. Sau 4 tu\u1ea7n nu\u00f4i c\u1ea5y, c\u00e2y c\u00f3 trung b\u00ecnh 7,2 r\u1ec5\/c\u00e2y, chi\u1ec1u d\u00e0i r\u1ec5 trung b\u00ecnh \u0111\u1ea1t 10,9 cm. K\u1ebft qu\u1ea3 n\u00e0y cho th\u1ea5y vi\u1ec7c l\u1ef1a ch\u1ecdn th\u00e0nh ph\u1ea7n m\u00f4i tr\u01b0\u1eddng ph\u00f9 h\u1ee3p c\u00f3 vai tr\u00f2 quan tr\u1ecdng trong n\u00e2ng cao ch\u1ea5t l\u01b0\u1ee3ng c\u00e2y gi\u1ed1ng tr\u01b0\u1edbc khi chuy\u1ec3n sang giai \u0111o\u1ea1n th\u00edch nghi ngo\u00e0i \u0111i\u1ec1u ki\u1ec7n <em>in vitro<\/em>.<\/p>\n<table id=\"undefined\" class=\"imgEditor\" border=\"0\">\n<tbody>\n<tr>\n<td><img decoding=\"async\" class=\"imgtelerik\" src=\"https:\/\/file.vnua.edu.vn\/data\/0\/images\/2026\/10\/21\/host\/image003.png?w=680\" alt=\"\" \/><\/td>\n<\/tr>\n<tr class=\"alt_imgEditor\">\n<td><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u1ede giai \u0111o\u1ea1n nh\u00e2n nhanh, m\u00f4i tr\u01b0\u1eddng MS b\u1ed5 sung 20 g\/l sucrose, 6,5 g\/l agar, 30 ml\/l n\u01b0\u1edbc d\u1eeba, 1,0 mg\/l Kinetin v\u00e0 1,0 mg\/l IBA \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 ph\u00f9 h\u1ee3p nh\u1ea5t trong c\u00e1c c\u00f4ng th\u1ee9c kh\u1ea3o s\u00e1t. Sau 8 tu\u1ea7n nu\u00f4i c\u1ea5y, h\u1ec7 s\u1ed1 nh\u00e2n \u0111\u1ea1t 15,75 ch\u1ed3i\/m\u1eabu, chi\u1ec1u cao ch\u1ed3i trung b\u00ecnh \u0111\u1ea1t 3,35 cm.<\/p>\n<p>\u0110\u1ed1i v\u1edbi giai \u0111o\u1ea1n t\u1ea1o r\u1ec5, m\u00f4i tr\u01b0\u1eddng MS b\u1ed5 sung 6,5 g\/l agar, 20 g\/l sucrose v\u00e0 0,7 g\/l than ho\u1ea1t t\u00ednh cho t\u1ef7 l\u1ec7 t\u1ea1o r\u1ec5 \u0111\u1ea1t 100%. Sau 4 tu\u1ea7n nu\u00f4i c\u1ea5y, c\u00e2y c\u00f3 trung b\u00ecnh 7,2 r\u1ec5\/c\u00e2y, chi\u1ec1u d\u00e0i r\u1ec5 trung b\u00ecnh \u0111\u1ea1t 10,9 cm. K\u1ebft qu\u1ea3 n\u00e0y cho th\u1ea5y vi\u1ec7c l\u1ef1a ch\u1ecdn th\u00e0nh ph\u1ea7n m\u00f4i tr\u01b0\u1eddng ph\u00f9 h\u1ee3p c\u00f3 vai tr\u00f2 quan tr\u1ecdng trong n\u00e2ng cao ch\u1ea5t l\u01b0\u1ee3ng c\u00e2y gi\u1ed1ng tr\u01b0\u1edbc khi chuy\u1ec3n sang giai \u0111o\u1ea1n th\u00edch nghi ngo\u00e0i \u0111i\u1ec1u ki\u1ec7n <em>in vitro<\/em>.<\/p>\n<table id=\"undefined\" class=\"imgEditor\" border=\"0\">\n<tbody>\n<tr>\n<td><img decoding=\"async\" class=\"imgtelerik\" src=\"https:\/\/file.vnua.edu.vn\/data\/0\/images\/2026\/10\/21\/host\/image005.png?w=680\" alt=\"\" \/><\/td>\n<\/tr>\n<tr class=\"alt_imgEditor\">\n<td><strong><em>H\u00ecnh \u1ea3nh lan<\/em><\/strong><strong><em> A. lylei<\/em><\/strong> <strong><em>nu\u00f4i c\u1ea5y in vitro\u1edfc\u00e1c giai \u0111o\u1ea1n kh\u00e1c nhau<\/em><\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>B\u00ean c\u1ea1nh giai \u0111o\u1ea1n nu\u00f4i c\u1ea5y trong ph\u00f2ng th\u00ed nghi\u1ec7m, nghi\u00ean c\u1ee9u c\u0169ng \u0111\u00e1nh gi\u00e1 gi\u00e1 th\u1ec3 ph\u1ee5c v\u1ee5 qu\u00e1 tr\u00ecnh sinh tr\u01b0\u1edfng c\u1ee7a c\u00e2y con trong v\u01b0\u1eddn \u01b0\u01a1m. K\u1ebft qu\u1ea3 cho th\u1ea5y h\u1ed7n h\u1ee3p gi\u00e1 th\u1ec3 g\u1ed3m \u0111\u1ea5t ph\u00f9 sa, tr\u1ea5u hun v\u00e0 ph\u00e2n chu\u1ed3ng theo t\u1ef7 l\u1ec7 1:1:1, v\u1edbi \u0111\u1ed9 \u1ea9m duy tr\u00ec \u1edf m\u1ee9c 80-90%, ph\u00f9 h\u1ee3p nh\u1ea5t trong c\u00e1c c\u00f4ng th\u1ee9c kh\u1ea3o s\u00e1t. Vi\u1ec7c l\u1ef1a ch\u1ecdn gi\u00e1 th\u1ec3 th\u00edch h\u1ee3p gi\u00fap t\u1ea1o \u0111i\u1ec1u ki\u1ec7n thu\u1eadn l\u1ee3i cho c\u00e2y con th\u00edch nghi v\u1edbi m\u00f4i tr\u01b0\u1eddng b\u00ean ngo\u00e0i, \u0111\u1ed3ng th\u1eddi g\u00f3p ph\u1ea7n n\u00e2ng cao hi\u1ec7u qu\u1ea3 c\u1ee7a quy tr\u00ecnh nh\u00e2n gi\u1ed1ng t\u1eeb nu\u00f4i c\u1ea5y m\u00f4 \u0111\u1ebfn giai \u0111o\u1ea1n ch\u0103m s\u00f3c t\u1ea1i v\u01b0\u1eddn \u01b0\u01a1m.<\/p>\n<p>Nh\u1eefng k\u1ebft qu\u1ea3 thu \u0111\u01b0\u1ee3c \u0111\u00e3 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c \u0111i\u1ec1u ki\u1ec7n ph\u00f9 h\u1ee3p cho nh\u1eefng giai \u0111o\u1ea1n ch\u00ednh trong quy tr\u00ecnh nh\u00e2n gi\u1ed1ng <em>in vitro<\/em> c\u00e2y lan kim tuy\u1ebfn t\u1eeb ch\u1ed3i n\u00e1ch, bao g\u1ed3m kh\u1eed tr\u00f9ng m\u1eabu, t\u1ea1o c\u1ee5m ch\u1ed3i, nh\u00e2n nhanh ch\u1ed3i, t\u1ea1o r\u1ec5 v\u00e0 th\u00edch nghi ngo\u00e0i \u0111i\u1ec1u ki\u1ec7n nu\u00f4i c\u1ea5y. \u0110\u00e2y l\u00e0 c\u01a1 s\u1edf khoa h\u1ecdc c\u00f3 \u00fd ngh\u0129a \u0111\u1ed1i v\u1edbi vi\u1ec7c ti\u1ebfp t\u1ee5c ho\u00e0n thi\u1ec7n quy tr\u00ecnh nh\u00e2n gi\u1ed1ng, n\u00e2ng cao hi\u1ec7u qu\u1ea3 t\u1ea1o c\u00e2y gi\u1ed1ng v\u00e0 ph\u1ee5c v\u1ee5 c\u00e1c nghi\u00ean c\u1ee9u ti\u1ebfp theo v\u1ec1 b\u1ea3o t\u1ed3n, ph\u00e1t tri\u1ec3n ngu\u1ed3n gen c\u00e2y lan kim tuy\u1ebfn.<\/p>\n<p>Nghi\u00ean c\u1ee9u \u0111\u1ed3ng th\u1eddi kh\u1eb3ng \u0111\u1ecbnh vai tr\u00f2 c\u1ee7a vi\u1ec7c l\u1ef1a ch\u1ecdn m\u00f4i tr\u01b0\u1eddng dinh d\u01b0\u1ee1ng, ch\u1ea5t \u0111i\u1ec1u h\u00f2a sinh tr\u01b0\u1edfng v\u00e0 gi\u00e1 th\u1ec3 ph\u00f9 h\u1ee3p trong t\u1eebng giai \u0111o\u1ea1n ph\u00e1t tri\u1ec3n c\u1ee7a c\u00e2y. K\u1ebft qu\u1ea3 l\u00e0 ngu\u1ed3n t\u00e0i li\u1ec7u tham kh\u1ea3o h\u1eefu \u00edch cho c\u00f4ng t\u00e1c nghi\u00ean c\u1ee9u, gi\u1ea3ng d\u1ea1y v\u00e0 \u1ee9ng d\u1ee5ng c\u00f4ng ngh\u1ec7 nu\u00f4i c\u1ea5y m\u00f4 trong l\u0129nh v\u1ef1c n\u00f4ng nghi\u1ec7p.<\/p>\n<p><em>Chi ti\u1ebft xem t\u1ea1i: <a href=\"https:\/\/vie.vjas.vn\/index.php\/vjasvn\/article\/view\/2608\/2408\">https:\/\/vie.vjas.vn\/index.php\/vjasvn\/article\/view\/2608\/2408<\/a><\/em><\/p>\n<p><strong><em>\u0110\u00e0o H\u01b0\u01a1ng &#8211; Nh\u00e0 xu\u1ea5t b\u1ea3n<\/em><\/strong><\/div>\n<p><!-- .vgblk-rw-wrapper --><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Lan kim tuy\u1ebfn (Anoectochilus lylei Rolfe ex Downie) l\u00e0 lo\u00e0i lan c\u00f3 gi\u00e1 tr\u1ecb, \u0111\u01b0\u1ee3c quan t\u00e2m trong nghi\u00ean c\u1ee9u v\u00e0 b\u1ea3o t\u1ed3n ngu\u1ed3n gen th\u1ef1c v\u1eadt. Vi\u1ec7c x\u00e2y d\u1ef1ng quy tr\u00ecnh nh\u00e2n gi\u1ed1ng hi\u1ec7u qu\u1ea3 c\u00f3 \u00fd ngh\u0129a quan tr\u1ecdng trong vi\u1ec7c ch\u1ee7 \u0111\u1ed9ng t\u1ea1o ngu\u1ed3n c\u00e2y gi\u1ed1ng, ph\u1ee5c v\u1ee5 c\u00f4ng t\u00e1c b\u1ea3o t\u1ed3n&#8230;<\/p>\n","protected":false},"author":3,"featured_media":991086,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-991085","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/crei.vnua.edu.vn\/index.php?rest_route=\/wp\/v2\/posts\/991085","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/crei.vnua.edu.vn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/crei.vnua.edu.vn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/crei.vnua.edu.vn\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/crei.vnua.edu.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=991085"}],"version-history":[{"count":1,"href":"https:\/\/crei.vnua.edu.vn\/index.php?rest_route=\/wp\/v2\/posts\/991085\/revisions"}],"predecessor-version":[{"id":991087,"href":"https:\/\/crei.vnua.edu.vn\/index.php?rest_route=\/wp\/v2\/posts\/991085\/revisions\/991087"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/crei.vnua.edu.vn\/index.php?rest_route=\/wp\/v2\/media\/991086"}],"wp:attachment":[{"href":"https:\/\/crei.vnua.edu.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=991085"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/crei.vnua.edu.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=991085"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/crei.vnua.edu.vn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=991085"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}